6GKR
Crystal structure of branched-chain amino acid aminotransferase from Thermobaculum terrenum in PLP-form (holo-form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.509, 146.610, 119.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 84.010 - 2.190 |
| R-factor | 0.1617 |
| Rwork | 0.159 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6GKP |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.604 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.010 | 2.260 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmeas | 0.172 | 0.884 |
| Number of reflections | 45692 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 98.3 | 99 |
| Redundancy | 4.4 | 4.2 |
| CC(1/2) | 0.990 | 0.661 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 7.5 | 293 | HEPES 0.1M pH 7.5; Sodium chloride 3.4M |
