6GKL

Translation initiation factor 4E in complex with beta-phosphorothioate trinucleotide mRNA 5' cap diastereomer 2 (m7GppSpApG D2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1
Unit cell lengths	38.170, 38.180, 147.020
Unit cell angles	88.80, 84.67, 76.13

Refinement procedure

Resolution	48.795 - 2.200
R-factor	0.2195
Rwork	0.218
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m7x
RMSD bond length	0.006
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.800	2.260
High resolution limit [Å]	2.200	2.200
Rmerge	0.124	0.415
Rmeas	0.145	0.555
Number of reflections	39200	2365
<I/σ(I)>	6.35	1.85
Completeness [%]	96.1	75.5
Redundancy	3.478	1.918
CC(1/2)	0.991	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	16% PEG 10000, 0.1M TrisHCl pH 8.0, 0.2M CH3COONH4