6GHY
Structure of Lytic Transglycosylase MltE inactive mutant E64Q from E.coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.972420 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 121.488, 181.138, 34.829 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.550 - 2.120 |
R-factor | 0.20489 |
Rwork | 0.203 |
R-free | 0.24435 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2y8p |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.550 | 2.190 |
High resolution limit [Å] | 2.120 | 2.120 |
Rpim | 0.047 | |
Number of reflections | 22063 | |
<I/σ(I)> | 11 | |
Completeness [%] | 98.1 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 8.4 | 291 | 26% Polyethylene Glycol 4000, 0.2 M magnesium chloride and 0.1 M TRIS HCl (pH 8.4) |