6GHY

Structure of Lytic Transglycosylase MltE inactive mutant E64Q from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.972420
Spacegroup name	C 2 2 21
Unit cell lengths	121.488, 181.138, 34.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 2.120
R-factor	0.20489
Rwork	0.203
R-free	0.24435
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y8p
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.550	2.190
High resolution limit [Å]	2.120	2.120
Rpim	0.047
Number of reflections	22063
<I/σ(I)>	11
Completeness [%]	98.1
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8.4	291	26% Polyethylene Glycol 4000, 0.2 M magnesium chloride and 0.1 M TRIS HCl (pH 8.4)