6GHR

cyanobacterial GAPDH with full-length CP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97951
Spacegroup name	P 21 21 2
Unit cell lengths	133.438, 146.964, 81.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.226 - 2.249
R-factor	0.1918
Rwork	0.190
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4boy
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.230	2.329
High resolution limit [Å]	2.249	2.249
Rmerge	0.099	0.463
Rmeas	0.116	0.565
Number of reflections	76310	7510
<I/σ(I)>	7.68	1.82
Completeness [%]	99.3	98.79
Redundancy	3.5	2.9
CC(1/2)	0.993	0.709

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	290	4% Tacsimate, pH 7 12% (w/v) PEG3350