6GHG
Variable heavy - variable light domain and Fab-arm CrossMabs with charged residue exchanges
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99993 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.560, 59.800, 79.090 |
| Unit cell angles | 99.49, 89.56, 92.61 |
Refinement procedure
| Resolution | 18.950 - 1.880 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.310 | 1.970 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 85296 | |
| <I/σ(I)> | 7.01 | 1.05 |
| Completeness [%] | 97.5 | |
| Redundancy | 3.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1M MES, pH 6.5, 20% PEG2000MME |
