6GHB
Crystal structure of Spx in complex with YjbH (oxidized)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.596, 88.704, 117.467 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.429 - 3.104 |
| R-factor | 0.226 |
| Rwork | 0.222 |
| R-free | 0.26590 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.107 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.429 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 16640 | 1613 |
| <I/σ(I)> | 10.97 | 1.68 |
| Completeness [%] | 99.2 | 98.59 |
| Redundancy | 4.1 | 4.1 |
| CC(1/2) | 0.994 | 0.609 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Na-HEPES pH 7.5, MgCl2 and PEG400 |
