6GG9
Crystal structures of a short blue light photoreceptor protein PpSB1-LOV mutant (dark state) - R61H/R66I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9772 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.700, 76.506, 83.279 |
| Unit cell angles | 90.00, 106.23, 90.00 |
Refinement procedure
| Resolution | 48.450 - 2.040 |
| R-factor | 0.1968 |
| Rwork | 0.195 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5j3w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1-2155_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 2.100 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.069 | |
| Rmeas | 0.078 | |
| Number of reflections | 39334 | 3076 |
| <I/σ(I)> | 11.2 | 1.7 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 4.4 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 292.15 | 0.2 MgCl2, 0.1M MES, 15% (w/v) PEG4000 |
