6GFQ

cyanobacterial GAPDH with NAD and CP12 bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	141.217, 141.217, 76.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.768 - 1.400
R-factor	0.1471
Rwork	0.146
R-free	0.17360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3zdf
RMSD bond length	0.005
RMSD bond angle	0.931
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.770	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.058	0.499
Rmeas	0.060	0.577
Number of reflections	147904	12946
<I/σ(I)>	19.71
Completeness [%]	98.1	86.93
Redundancy	10.5	3.6
CC(1/2)	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	4% Tacsimate, pH 7, 8 % PEG3350