6GFO

cyanobacterial GAPDH with full-length CP12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 2
Unit cell lengths	136.446, 141.049, 81.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.627 - 2.100
R-factor	0.1731
Rwork	0.171
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4boy
RMSD bond length	0.007
RMSD bond angle	0.905
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.630	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.139	0.496
Rmeas	0.151	0.542
Rpim	0.060
Number of reflections	92479	9129
<I/σ(I)>	6.9	2.45
Completeness [%]	99.9	99.91
Redundancy	6.3	6.2
CC(1/2)	0.991	0.905

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		290	1% Tryptone, 0.001 M Azide, 0.005 M Azide, 12 % PEG3350