6GFN
METTL16 MTase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-02 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 92.360, 92.360, 180.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 82.230 - 2.860 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gfk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS (Nov 11, 2017 (BUILT 20171111)) |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.240 | 3.200 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.177 | 1.320 |
| Rmeas | 0.192 | 1.420 |
| Rpim | 0.073 | 0.500 |
| Number of reflections | 5724 | 286 |
| <I/σ(I)> | 8 | 1.8 |
| Completeness [%] | 93.1 | 78.7 |
| Redundancy | 6.9 | 7.8 |
| CC(1/2) | 0.996 | 0.590 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M di-sodium tartrate and 20% (w/v) PEG 3350 |
