6GFJ
Structure of RIP2 CARD domain fused to crystallisable MBP tag
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 89.460, 121.570, 123.160 |
| Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
| Resolution | 46.980 - 3.300 |
| R-factor | 0.23613 |
| Rwork | 0.235 |
| R-free | 0.26569 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ifp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.053 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.980 | 3.380 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmeas | 0.190 | 1.370 |
| Number of reflections | 37320 | |
| <I/σ(I)> | 5.45 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.25 M NaNO3, and 22% (w/v) PEG 3350 |
