6GF8
Molecular basis of egg coat filament cross-linking: structure of the glycosylated ZP1 ZP-N1 domain homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.774, 125.734, 105.001 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.725 - 3.100 |
| R-factor | 0.2127 |
| Rwork | 0.210 |
| R-free | 0.23520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Monomer 6GF6 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.657 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.740 | 3.240 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmeas | 0.093 | 1.114 |
| Rpim | 0.043 | 0.520 |
| Number of reflections | 11119 | 1328 |
| <I/σ(I)> | 9.5 | 1 |
| Completeness [%] | 99.1 | 96.9 |
| Redundancy | 4.1 | 4.2 |
| CC(1/2) | 1.000 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 29% (V/V) MPD, 0.1 M MES PH 6.5 |
