6GF6

Molecular basis of egg coat filament cross-linking: high-resolution structure of the partially deglycosylated ZP1 ZP-N1 domain homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.7710
Spacegroup name	P 41 21 2
Unit cell lengths	75.980, 75.980, 101.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.966 - 2.300
R-factor	0.1958
Rwork	0.192
R-free	0.22920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Partially refined model derived from SAD phasing of a gold derivative
RMSD bond length	0.005
RMSD bond angle	0.738
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((dev_3409: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.990	2.400
High resolution limit [Å]	2.300	2.300
Rmeas	0.116	1.498
Rpim	0.026	0.495
Number of reflections	13550	1422
<I/σ(I)>	22.2	1.4
Completeness [%]	98.0	84.6
Redundancy	19.7	8.3
CC(1/2)	1.000	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.6 M LiCl, 0.1 M tri-sodium citrate pH 5.5