6GF0

Lysozyme structure determined from SFX data using a Sheet-on-Sheet chipless chip

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2017-12-18
Detector	MPCCD
Wavelength(s)	1.770
Spacegroup name	P 43 21 2
Unit cell lengths	79.100, 79.100, 39.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.550 - 2.070
R-factor	0.20034
Rwork	0.199
R-free	0.23483
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4n5r
RMSD bond length	0.006
RMSD bond angle	0.962
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.200
High resolution limit [Å]	2.070	2.070
Number of reflections	7417
<I/σ(I)>	10.3	2.8
Completeness [%]	100.0	100
Redundancy	283	42
CC(1/2)	0.986	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	20 % NaCl, 6 % PEG 6000, 0.1 M sodium acetate pH 3.0