6GEZ
THE STRUCTURE OF TWITCH-2B N532F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.619, 157.472, 169.419 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 115.340 - 2.470 |
| R-factor | 0.208 |
| Rwork | 0.207 |
| R-free | 0.23400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gel |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.708 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 115.340 | 2.570 |
| High resolution limit [Å] | 2.470 | 2.470 |
| Rmerge | 0.060 | 0.520 |
| Number of reflections | 54945 | |
| <I/σ(I)> | 14.95 | 1.95 |
| Completeness [%] | 97.5 | 80.9 |
| Redundancy | 6.36 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 0.2 M SODIUM FORMIATE, PH 7.0, 5 MM CALCIUM CHLORIDE, 20 % PEG3350 |
