6GEH
Structure and reactivity of a siderophore-interacting protein from the marine bacterium Shewanella reveals unanticipated functional versatility.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.098, 77.753, 44.917 |
Unit cell angles | 90.00, 109.64, 90.00 |
Refinement procedure
Resolution | 28.620 - 1.150 |
R-factor | 0.1463 |
Rwork | 0.145 |
R-free | 0.17350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4yhb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.384 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.620 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Number of reflections | 86403 | |
<I/σ(I)> | 18.4 | |
Completeness [%] | 99.0 | |
Redundancy | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | PEG 3350, Magnesium formate dihydrate |