6GEE
TEAD4 (216-434);E263A COMPLEXED WITH YAP PEPTIDE (60-100); S94A AND MYRISTOATE (COVALENTLY BOUND) AT 1.96A (P41212 CRYSTAL FORM); MYRISTOYLATION WAS DONE BY ADDING MYR-COA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99994 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.315, 59.315, 158.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.790 - 1.960 |
| R-factor | 0.2176 |
| Rwork | 0.216 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ge3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.790 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.116 | 0.982 |
| Number of reflections | 21126 | |
| <I/σ(I)> | 16.4 | 3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 12.7 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 17% PEG 10000, 0.1M ammonium acetate, 0.1M Bis-TRIS |
