6GE9
Structure of Mycobacterium tuberculosis GlmU bound to Glc-1P and Ac-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 109.753, 109.753, 364.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.126 - 2.260 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.27650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g87 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.567 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.710 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.070 | 1.070 |
| Rpim | 0.460 | |
| Number of reflections | 40178 | 3861 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 98.3 | 96.6 |
| Redundancy | 6.5 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.05 M ADA pH 6.8, 5.7% PEG 550 MME, 32.1% PEG 200 |
