6GDS
Holliday Junctions formed from Telomeric DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.92016 |
| Spacegroup name | P 1 |
| Unit cell lengths | 23.334, 33.183, 41.303 |
| Unit cell angles | 99.27, 90.00, 110.59 |
Refinement procedure
| Resolution | 15.740 - 2.950 |
| R-factor | 0.2441 |
| Rwork | 0.242 |
| R-free | 0.28630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hs1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.457 |
| Data reduction software | CrysalisPro (1.171.37.34) |
| Data scaling software | Aimless (0.6.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.8.0222) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 15.740 | 15.360 | 2.950 |
| High resolution limit [Å] | 2.800 | 8.860 | 2.800 |
| Rmerge | 0.114 | 0.094 | 0.336 |
| Rmeas | 0.161 | 0.133 | 0.475 |
| Rpim | 0.114 | 0.094 | 0.336 |
| Total number of observations | 101 | 726 | |
| Number of reflections | 2615 | 58 | 408 |
| <I/σ(I)> | 5.6 | 11.3 | 1.5 |
| Completeness [%] | 92.9 | 63 | 96.5 |
| Redundancy | 1.8 | 1.7 | 1.8 |
| CC(1/2) | 0.947 | 0.943 | 0.952 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | 25 mM Potassium Chloride, 10 mM Potassium Cacodylate, 50mM Magnesium Chloride, 20mM Calcium Chloride, 35% v/v (+/-)-2-Methyl-2,4-Pentanediol. |
