6GDH

Holliday Junctions formed from Telomeric DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	Agilent SuperNova
Temperature [K]	105
Detector technology	CCD
Collection date	2014-11-12
Detector	AGILENT TITAN CCD
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	23.733, 33.161, 83.467
Unit cell angles	99.87, 90.07, 110.94

Refinement procedure

Resolution	22.510 - 2.850
R-factor	0.2298
Rwork	0.228
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hs1
RMSD bond length	0.008
RMSD bond angle	1.552
Data reduction software	CrysalisPro (1.171.36.32)
Data scaling software	Aimless (0.6.3)
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	22.510	22.510	2.950
High resolution limit [Å]	2.800	8.860	2.800
Rmerge	0.059	0.026	0.447
Rmeas	0.070	0.031	0.555
Rpim	0.037	0.016	0.324
Total number of observations	19984
Number of reflections	5625	169	813
<I/σ(I)>	11.4
Completeness [%]	97.8	88.9	93.6
Redundancy	3.6	3.9	2.6
CC(1/2)	0.998	0.999	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	285	25 mM potassium chloride, 10 mM potassium cacodylate 50mM for magnesium chloride, 20mM for calcium chloride, MPD 32% to 38%