6GD1
Structure of HuR RRM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.042 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.920, 67.660, 70.140 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 8.980 - 2.010 |
| R-factor | 0.17943 |
| Rwork | 0.176 |
| R-free | 0.24417 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2trx |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.819 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.990 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmeas | 0.100 | 0.850 |
| Number of reflections | 26790 | 1988 |
| <I/σ(I)> | 12.48 | 2.2 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 6.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23% (w/v) PEG 2000 MME, 0.1 M potassium thiocyanate |
