6GCE
DNA binding domain of restriction endonuclease McrBC in complex with 5-formylcytosine DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.001 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.175, 69.988, 144.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.089 - 1.600 |
| R-factor | 0.1712 |
| Rwork | 0.167 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ssc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.046 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.090 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.448 |
| Number of reflections | 92903 | 9718 |
| <I/σ(I)> | 17.15 | 2.39 |
| Completeness [%] | 99.6 | 99.92 |
| Redundancy | 6.3 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M LiCl, 22% PEG4000 |
