6GC1

Crystal structure of Trx-like and NHL repeat containing domains of human NHLRC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-30
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 1 21 1
Unit cell lengths	103.284, 103.812, 114.978
Unit cell angles	90.00, 100.77, 90.00

Refinement procedure

Resolution	47.770 - 2.700
R-factor	0.248
Rwork	0.246
R-free	0.28720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hyx
RMSD bond length	0.009
RMSD bond angle	1.375
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.770	47.770	2.770
High resolution limit [Å]	2.700	12.070	2.700
Rmerge	0.130	0.043	1.088
Rmeas	0.147	0.049	1.220
Number of reflections	65063	762	4852
<I/σ(I)>	8.99	24.58	1.29
Completeness [%]	98.9	96.9	99.5
Redundancy	4.719	4.331	4.892
CC(1/2)	0.994	0.997	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M BisTris Propane pH 6.5, 0.2 M K/Na Tartrate, 18% PEG 3350