6GAY

A fast recovering full-length LOV protein (DsLOV) from the marine phototrophic bacterium Dinoroseobacter shibae (Dark state) - M49I mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	56.675, 56.675, 156.383
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.889 - 1.860
R-factor	0.1679
Rwork	0.166
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kuk
RMSD bond length	0.020
RMSD bond angle	1.624
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1-2155_1692: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.890	1.900
High resolution limit [Å]	1.860	1.860
Rmerge	0.061
Rmeas	0.065
Number of reflections	22330	1358
<I/σ(I)>	23.2	2.2
Completeness [%]	99.9	100
Redundancy	9.2	9.2
CC(1/2)		0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	292.15	0.1 M Na-acetate, 0.1 M Ammonium sulfate, 0.3 M Sodium formate, 3 % PGA-LM, 5 % (w/v) PEG 8000