6GAU

Extremely 'open' clamp structure of DNA gyrase: role of the Corynebacteriales GyrB specific insert

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.950
Spacegroup name	P 1
Unit cell lengths	91.523, 96.782, 105.791
Unit cell angles	75.64, 64.44, 65.80

Refinement procedure

Resolution	48.350 - 3.300
R-factor	0.177
Rwork	0.173
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ifz 3ig0 3zkb
RMSD bond length	0.010
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	48.350
High resolution limit [Å]	3.300
Rmerge	0.236
Number of reflections	44305
<I/σ(I)>	7
Completeness [%]	98.2
Redundancy	3.99
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Sodium Acetate 100 mM MES pH 6.5 26% PEG 400 25% EG 10 mM MgCl2