6G9X

Crystal structure of a MFS transporter at 2.54 Angstroem resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	106.765, 200.539, 64.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.790 - 2.300
R-factor	0.2196
Rwork	0.217
R-free	0.25410
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.156
Data reduction software	XDS (Jun 1, 2017 (BUILT 20170615)
Data scaling software	autoPROC (1.0.5 (20170920))
Phasing software	CRANK2 (2.0.153)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.260	2.540
High resolution limit [Å]	2.300	2.300
Rmeas	0.070	1.700
Rpim	0.030	0.700
Number of reflections	34625	1731
<I/σ(I)>	19.9	1.7
Completeness [%]	94.0	87.1
Redundancy	12.9	11.2
CC(1/2)	0.999	0.594

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	zinc bromide, HEPES, jeffamine ED-2003