6G8Y
Crystal Structures of the Single PDZ Domains from GRASP65 and their Interaction with the Golgin GM130
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-01-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.443, 53.443, 50.923 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.720 - 1.400 |
| R-factor | 0.16519 |
| Rwork | 0.163 |
| R-free | 0.21006 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.452 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmeas | 0.042 | 0.740 |
| Number of reflections | 16914 | 1228 |
| <I/σ(I)> | 22.96 | 2.82 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.5 | 6.4 |
| CC(1/2) | 0.999 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.0 M NaCl, 100 mM Bis-Tris, pH 5.5 |
