6G8A
Lysozyme solved by Native SAD from a dataset collected in 5 seconds at 1 A wavelength with JUNGFRAU detector
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-16 |
| Detector | PSI JUNGFRAU 1M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.903, 78.903, 36.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.262 - 1.143 |
| R-factor | 0.1132 |
| Rwork | 0.112 |
| R-free | 0.13040 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXCD |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.450 | 1.210 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmeas | 0.037 | 0.220 |
| Number of reflections | 77341 | 9912 |
| <I/σ(I)> | 41.82 | 4.95 |
| Completeness [%] | 95.8 | 75.8 |
| Redundancy | 15.1 | 3.4 |
| CC(1/2) | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 5% PEG MME 5000, 2 M NaCl, 50 mM Sodium Acetate pH 4.5, 25% 480 ethylene glycol |
