6G7Z
Lariat-capping ribozyme with a shortened DP2 stem loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-10 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.945, 88.791, 110.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.395 - 3.336 |
| R-factor | 0.238474450676 |
| Rwork | 0.235 |
| R-free | 0.30422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p8z |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.729 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXS |
| Refinement software | REFMAC (1.11rc1_2513) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.400 | 3.818 |
| High resolution limit [Å] | 3.330 | 3.336 |
| Number of reflections | 108094 | 2173 |
| <I/σ(I)> | 8.37 | |
| Completeness [%] | 94.1 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 200 mM NaCl 100 mM HEPES pH 7.5 5-25% w/v PEG 3350 |
