6G6S

Crystal structure of human Acinus RNA recognition motif domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.918410
Spacegroup name	P 21 21 21
Unit cell lengths	30.359, 67.911, 80.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.070 - 1.650
R-factor	0.1872
Rwork	0.185
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2x1f
RMSD bond length	0.017
RMSD bond angle	1.780
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.070	80.070	1.740
High resolution limit [Å]	1.650	4.910	1.650
Rmeas	0.071	0.024	0.864
Number of reflections	19003	864	3228
<I/σ(I)>	16.3	46.1	1.97
Completeness [%]	91.6	97.7	98.5
Redundancy	4.76
CC(1/2)	0.999	0.999	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.2 M Ammonium acetate, 100 mM Tris-HCl pH 8.5, 25% w/v Polyethylene glycol 3350