6G64
MamM CTD H264A-E289A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | Cu FINE FOCUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-06-06 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.112, 95.086, 53.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.540 - 1.900 |
| R-factor | 0.2084 |
| Rwork | 0.205 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3w5x |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.040 | 0.033 | 0.240 |
| Rmeas | 0.042 | 0.035 | 0.273 |
| Rpim | 0.014 | 0.012 | 0.125 |
| Number of reflections | 7559 | 432 | 338 |
| <I/σ(I)> | 16.2 | ||
| Completeness [%] | 97.2 | 97.7 | 88.9 |
| Redundancy | 8.5 | 8.7 | 4 |
| CC(1/2) | 0.999 | 0.892 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium sulfate, 0.1 M BIS-TRIS pH=5.5, 22% PEG 3350, 12.7 uM MnCl2 |
