6G5O
The structure of a carbohydrate active P450
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.280, 68.152, 199.728 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.860 - 2.250 |
| R-factor | 0.20708 |
| Rwork | 0.205 |
| R-free | 0.24802 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5l90 4ubs 1cl6 and 3ejd |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.556 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.870 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 41766 | 57238 |
| <I/σ(I)> | 14.4 | 3.3 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 20% PEG 3350, 150 mM NaCl |
