6G4R
Corynebacterium glutamicum OxyR C206S mutant, H2O2-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.216, 56.991, 154.182 |
| Unit cell angles | 90.00, 98.02, 90.00 |
Refinement procedure
| Resolution | 76.340 - 2.620 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 153.000 | 153.000 | 2.690 |
| High resolution limit [Å] | 2.620 | 11.720 | 2.620 |
| Rmerge | 0.246 | 0.103 | 2.039 |
| Rmeas | 0.268 | 0.113 | 2.217 |
| Rpim | 0.105 | 0.046 | 0.861 |
| Total number of observations | 241902 | 2759 | 17931 |
| Number of reflections | 37454 | 463 | 2762 |
| <I/σ(I)> | 5.3 | 12.7 | 1.4 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 6.5 | 6 | 6.5 |
| CC(1/2) | 0.987 | 0.983 | 0.336 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 283 | PEG 4000, 2-(N-morpholino)ethanesulfonic acid (MES), lithium sulfate, TCEP |
