6G4B
Crystal structure of the omega transaminase from Pseudomonas jessenii in the apo form, crystallized from succinate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-03-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 98.240, 98.240, 119.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.900 - 1.800 |
| R-factor | 0.1418 |
| Rwork | 0.141 |
| R-free | 0.16575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4grx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.900 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.038 | 0.402 |
| Number of reflections | 104659 | 4975 |
| <I/σ(I)> | 17 | 2 |
| Completeness [%] | 99.8 | 95.3 |
| Redundancy | 7.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 1.0 M Sodium succinate, pH 7.5 |
