6G43

Crystal structure of SeMet-labeled mavirus major capsid protein lacking the C-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	135.350, 135.300, 136.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.000 - 2.400
R-factor	0.2145
Rwork	0.213
R-free	0.23360
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.104
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.000	48.000	2.500
High resolution limit [Å]	2.400	20.000	2.400
Rmerge	0.118	0.041	0.609
Rmeas	0.122	0.043	0.633
Number of reflections	188659	309	21723
<I/σ(I)>	20.39	56.58	5.51
Completeness [%]	100.0	93.4	100
Redundancy	13.816	14.269	13.323
CC(1/2)	0.999	0.999	0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	0.1 M tri-sodium citrate, 1.2 - 1.7 M ammonium sulfate