6G3P
X-ray structure of seleno-methionine labelled NSD3-PWWP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-02 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.040, 88.550, 77.970 |
| Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
| Resolution | 17.280 - 2.800 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.280 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.155 | 0.467 |
| Rmeas | 0.183 | 0.548 |
| Number of reflections | 29364 | 5430 |
| <I/σ(I)> | 7.79 | 2.8 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 3.509 | 3.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 50 mM TRIS pH 9.0, 23% PEG3350 |
