6G3J
MHC A02 Allele presenting MTSAIGILVP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.96859 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.940, 50.280, 119.070 |
| Unit cell angles | 90.00, 122.86, 90.00 |
Refinement procedure
| Resolution | 41.050 - 2.450 |
| R-factor | 0.22569 |
| Rwork | 0.223 |
| R-free | 0.27021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5eu5 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.900 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.050 | 2.510 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.104 | 1.155 |
| Rmeas | 0.123 | 1.354 |
| Number of reflections | 37034 | 2764 |
| <I/σ(I)> | 9.1 | 1 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.993 | 0.594 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 |
