6G2X

Crystal structure of the p97 D2 domain in a helical split-washer conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.92
Spacegroup name	P 61
Unit cell lengths	93.282, 93.282, 65.997
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.530 - 2.078
R-factor	0.2067
Rwork	0.204
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ftj
RMSD bond length	0.008
RMSD bond angle	1.003
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.530	2.153
High resolution limit [Å]	2.078	2.078
Number of reflections	19706
<I/σ(I)>	17.74
Completeness [%]	99.6
Redundancy	10.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.08M L-Na-Glutamate; 0.08M Alanine (racemic); 0.08M Glycine; 0.08M Lysine HCl (racemic); 0.08M Serine (racemic), 0.08M Tris, 0.08M BICINE, 10% v/v MPD; 10% PEG 1000; 10% w/v PEG 3350 at pH 8.5