6G2V
Crystal structure of the p97 D2 domain in a helical split-washer conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | P 61 |
Unit cell lengths | 90.140, 90.140, 65.210 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.070 - 1.903 |
R-factor | 0.2 |
Rwork | 0.197 |
R-free | 0.23670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ftj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.144 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1932) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.070 | 45.070 |
High resolution limit [Å] | 1.903 | 1.903 |
Number of reflections | 45713 | |
<I/σ(I)> | 22.7 | |
Completeness [%] | 98.6 | |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.08M L-Na-Glutamate; 0.08M Alanine (racemic); 0.08M Glycine; 0.08M Lysine HCl (racemic); 0.08M Serine (racemic), 0.08M Tris, 0.08M BICINE, 10% v/v MPD; 10% PEG 1000; 10% w/v PEG 3350 at pH 8.5 |