6G2K
Structure of HuR RRM3 in complex with RNA (UUUUUU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-19 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.310, 79.740, 51.070 |
| Unit cell angles | 90.00, 93.14, 90.00 |
Refinement procedure
| Resolution | 8.980 - 2.010 |
| R-factor | 0.1719 |
| Rwork | 0.169 |
| R-free | 0.23489 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.767 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.990 | 2.060 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmeas | 0.137 | 0.965 |
| Number of reflections | 18274 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M TRIS pH 8.5 and 2.25 M ammonium sulphate |
