6G28
Human [protein ADP-ribosylargenine] hydrolase ARH1 in complex with ADP-ribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9760 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.102, 66.687, 83.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.090 - 1.230 |
| R-factor | 0.14566 |
| Rwork | 0.145 |
| R-free | 0.15744 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hfw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.527 |
| Data reduction software | xia2 |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.690 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.059 | 0.262 |
| Rmeas | 0.064 | 0.295 |
| Rpim | 0.025 | 0.132 |
| Number of reflections | 103868 | 4932 |
| <I/σ(I)> | 21.7 | 5.5 |
| Completeness [%] | 99.6 | 97.1 |
| Redundancy | 12.2 | 9.3 |
| CC(1/2) | 0.998 | 0.981 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 200 mM magnesium formate, 20 % (w/v) PEG3350 |
