6G26
The crystal structure of the Burkholderia pseudomallei HicAB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-09-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.140, 74.190, 85.310 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 34.020 - 2.490 |
| R-factor | 0.18905 |
| Rwork | 0.187 |
| R-free | 0.23254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6g1n |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.150 | 2.530 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.111 | 1.246 |
| Rmeas | 0.120 | 1.349 |
| Number of reflections | 35696 | |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.997 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.1 M MES pH 6.5 0.2 M NH4S04 16% (w/v) PEG 5000 MME 25% (v/v) glycerol |
