6G1Z
Crystal structure of a fluorescence optimized bathy phytochrome PAiRFP2 derived from wild-type Agp2 in its Pfr state.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 182.377, 182.377, 179.883 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.730 - 2.030 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6g1y |
| RMSD bond length | 0.013 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.730 | 2.140 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rpim | 0.040 | 0.417 |
| Number of reflections | 113144 | |
| <I/σ(I)> | 15.4 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.3 | 13.6 |
| CC(1/2) | 0.999 | 0.592 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 279 | (NH4)2SO4, VAPOR DIFFUSION, TEMPERATURE 279K |
