6G1T
TraN, a repressor of an Enterococcus conjugative type IV secretion system
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.770, 44.170, 56.460 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 41.870 - 1.930 |
| Rwork | 0.181 |
| R-free | 0.21250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p0z |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.870 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.080 | |
| Number of reflections | 22900 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 96.7 | |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 293 | precipitant solution: 0.2 M Ammonium citrate tribasic, pH 7.0, 20 % (w/v) PEG 3,350 protein buffer: 25 mM HEPES pH 7.6, 75 mM Na2SO4 |
