6G1P
Apo form of ADP-ribosylserine hydrolase ARH3 of Latimeria chalumnae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.957, 99.882, 107.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 73.210 - 1.550 |
| R-factor | 0.14571 |
| Rwork | 0.145 |
| R-free | 0.16336 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2foz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.549 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.880 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.051 | 0.656 |
| Rmeas | 0.056 | 0.780 |
| Rpim | 0.022 | 0.415 |
| Number of reflections | 102142 | 4969 |
| <I/σ(I)> | 22.8 | 2.6 |
| Completeness [%] | 100.0 | 99.4 |
| Redundancy | 10.9 | 6.7 |
| CC(1/2) | 0.999 | 0.775 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 200 mM ammonium acetate, 21 % (w/v) PEG4000, 100 mM citrate pH 5.5 |
