6G1O
Structure of Pseudomonas aeruginosa Isocitrate Lyase, ICL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-13 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.943, 116.023, 128.533 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.491 - 1.882 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i4e |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.492 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmeas | 0.027 | 0.387 |
| Number of reflections | 48981 | 3287 |
| <I/σ(I)> | 1.08 | |
| Completeness [%] | 99.4 | 92.1 |
| Redundancy | 12.4 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | HEPES 0.1 M pH 5, CaCl2 0.1 M, 20% (w/v) PEG 6000, glycerol 5%, glyoxylate 1 mM and 2% thymol |
