6G0Z

Crystal structure of GDP bound RbgA from S. aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	71.932, 77.812, 124.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.649 - 2.150
R-factor	0.1839
Rwork	0.182
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1puj
RMSD bond length	0.009
RMSD bond angle	1.212
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.650	2.230
High resolution limit [Å]	2.150	2.150
Number of reflections	73381
<I/σ(I)>	2.45
Completeness [%]	99.8
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Lithium sulfate, 0.1 M MES pH 6.0, 35% (v/v) MPD