6FZV

Crystal structure of the metalloproteinase enhancer PCPE-1 bound to the procollagen C propeptide trimer (short)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9282
Spacegroup name	P 21 21 21
Unit cell lengths	89.090, 144.120, 158.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.370 - 2.700
R-factor	0.2275
Rwork	0.226
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ae2
RMSD bond length	0.003
RMSD bond angle	0.737
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.400	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.087	1.940
Number of reflections	56733
<I/σ(I)>	17.1	1.2
Completeness [%]	99.9	99.9
Redundancy	13.1	13.7
CC(1/2)	0.998	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20 mg/ml protein in 20 mM HEPES pH 7.5, 180 mM NaCl, 2.5 mM calcium chloride; 0.2 M potassium citrate, 16% PEG3350