6FZU
RORGT (264-518;C455S) IN COMPLEX WITH THE FRAGMENT ("CPD-1") AND RIP140 PEPTIDE AT 1.80A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.918, 67.874, 82.451 |
| Unit cell angles | 90.00, 101.85, 90.00 |
Refinement procedure
| Resolution | 19.800 - 1.800 |
| R-factor | 0.2166 |
| Rwork | 0.215 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nti |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.068 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.060 | 0.559 |
| Rmeas | 0.072 | 0.699 |
| Number of reflections | 46451 | |
| <I/σ(I)> | 11.8 | 2.2 |
| Completeness [%] | 96.9 | 93.1 |
| Redundancy | 3.1 | 2.6 |
| CC(1/2) | 0.997 | 0.652 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 22% PEG3350, 0.2M ammonium acetate, 0.1M bis-TRIS |
