6FZS
Crystal structure of Smad5-MH1 bound to the GGCGC site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-04 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.764, 73.374, 89.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.770 - 2.310 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kmp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.010 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.960 | 2.481 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Rpim | 0.052 | 0.555 |
| Number of reflections | 16675 | 834 |
| <I/σ(I)> | 11.4 | 1.5 |
| Completeness [%] | 92.4 | 44.6 |
| Redundancy | 6.1 | 6.4 |
| CC(1/2) | 0.998 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2 M NaF, 0.1 M bis-tris propane pH 7.5, 20% PEG3350 |
